Automated Gene Detection Using the Oligonucleotide Ligation Assay
Routine DNA analysis is assuming an increasing importance in clinical medicine as well as in basic research. The expanding field of DNA diagnostics will take advantage of the growing understanding of the molecular basis of human disease. Similarly, extensive studies of the structure of large genomes require efficient analytical techniques. In this chapter a strategy will be described that permits rapid distinction between alternate DNA sequences under standardized conditions. Applications of the technique includes screening for mutant gene sequences and genetic linkage analysis. The analysis may be performed manually or by a laboratory workstation resulting in a generally applicable automated gene analysis technique.
- A History of Association Mapping
- Direct Imaging of siRNA Electrotransfer at the Single-Cell Level
- DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins
- Signal Processing and the Design of Microarray Time-Series Experiments
- Sequential Deletions of Single-Stranded DNA: “Cyclone Sequencing”
- Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila
- Novel Approach for the Development of New Antibodies Directed Against Transposase-Derived Proteins Encoded by Human Neogenes
- Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica
- PRINS for the Detection of Gene Deletions in Cancer
- Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products