Comparative Genomic Hybridization: Microarray Design and Data Interpretation
Microarray-based Comparative Genomic Hybridization (array-CGH) has been applied for a decade to screen for submicroscopic DNA gains and losses in tumor and constitutional DNA samples. This method has become increasingly flexible with the integration of new biological resources generated by genome sequencing projects. In this chapter, we describe alternative strategies for whole genome screening and high resolution breakpoint mapping of copy number changes by array-CGH, as well as tools available for accurate analysis of array-CGH experiments. Although most methods listed here have been designed for microarrays comprising large-insert clones, they can be adapted easily to other types of microarray platforms, such as those constructed from printed or synthesized oligonucleotides.
- Purification of Baculovirus-Expressed Human DNA Topoisomerase I
- Site-Specific Chromosomal Integration Mediated by C31 Integrase
- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Lig
- Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein
- A T-Linker Strategy for Modification and Directional Cloning of PCR Products
- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
- Systemic Delivery of Antisense Oligomer in Animal Models and Its Implications for Treating DMD
- In-Depth Query of Large Genomes Using Tiling Arrays
- Proteomics-Based Method for Risk Assessment of Peroxisome Proliferating Pollutants in the Marine Environment
- Fluorochrome-Labeled Inhibitors of Caspases: Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis