Inverse PCR (IPCR) for Obtaining Promoter Sequence
Obtaining the promoter sequence for a gene when only the cDNA sequence is available can be an arduous task, especially because genomic DNA libraries usually in λ phage vectors need to be screened. An easier method to obtain promoter sequence without the need for libraries is to use the technique of inverse polymerase chain reaction (IPCR) (1 ,2 ). IPCR leads to the amplification of previously unknown sequences because the primers that initially face away from each other on the linear template can be made to face each other as in normal PCR following circularization of the template (Fig. 1 ). Further amplification with nested primers ensures the integrity of the final product, which can be sequenced directly.
- MIicroGenie: Shotgun DNA Sequencing
- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
- Long-Range PCR
- Activity Assays for Poly-ADP Ribose Polymerase
- Progress in the Therapeutic Applications of siRNAs Against HIV-1
- Analysis of In Vivo Methylation
- Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution
- Embryonic Stem Cells in the Study of Hematopoiesis
- Production of Chimeras Derived from Murine Embryonic Stem Cells
- Uses of Microarray Platforms in Cancer: A Correlative Study Between Genomic Copy Number Changes and Their Expression at mRNA and