Introduction to Animal DNA Barcoding Protocols
Procedures and protocols common to many DNA barcoding projects are summarized. Planning for any project should emphasize front-end procedures, especially the “genetic lockdown” of collected materials for downstream genetic procedures. Steps further into the DNA barcoding process chain, such as sequencing, data processing, and other back-end functions vary slightly, if at all, among projects and are presented elsewhere in the volume. Point-of-collection sample and tissue handling and data/metadata handling are stressed. Specific predictions of the future workflows and mechanics of DNA barcoding are difficult, so focus is on that which most or all future methods and technologies will surely share.
- RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts
- Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis
- Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device
- Comparing Microarray Studies
- Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
- Pyrosequencing Applications
- The Chemical Cleavage of Mismatch for the Detection of Mutations in Long DNA Fragments
- Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination
- Nested RT-PCR in a Single Closed Tube
- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization