Detection of Epigenetic Alterations Using Tiling Arrays
The epigenetic status of cancer cells is a consequence of the neoplastic transformation of their normal counterpart. Epigenetic changes directly influence gene expression and chromatin organization, which consequently leads to escape from the tumor-suppression mechanisms. Global mapping for specific epigenetic modifications (e.g., DNA methylation) of the entire genome is required to reveal epigenetic hotspots associated with a cancer type/stage. DNA tiling arrays may be applied for genome-wide analysis of different epigenetic marks. Tiling arrays are high-density DNA microarrays that can be custom-made to survey regions of interest (e.g., gene promoters) or permit whole-genome analysis. To identify the genomic alterations associated with testicular cancers we used tiling arrays to profile their methylome. We successfully identified numerous epigenetically modified loci that arose as a consequence of tumor progression.
- Single-Marker Family-Based Association Analysis Conditional on Parental Information
- Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes
- Tissue Microdissection
- Minigenes to Confirm Exon Skipping Mutations
- Overexpression and Purification of Bacterial Topoisomerase IV
- Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization
- Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of ProteinDNA Complexes
- Oligonucleotide Primed in Situ DNA Synthesis (PRINS): An Alternative to in Situ Hybridization for Gene Mapping and the Investiga
- Rationale for Developing New Virus Vectors to Analyze Gene Function in Grasses Through Virus-Induced Gene Silencing
- M13 Cloning Vehicles: Their Contribution to DNA Sequencing