DNA Barcodes: Methods and Protocols
关键词: dna barcodes methods protocols来源: 互联网
DNA barcoding, a new method for the quick identification of any species based on extracting a DNA sequence from a tiny tissue sample of any organism, is now being applied to taxa across the tree of life. As a research tool for taxonomists, DNA barcoding assists in identification by expanding the ability to diagnose species by including all life history stages of an organism. As a biodiversity discovery tool, DNA barcoding helps to flag species that are potentially new to science. As a biological tool, DNA barcoding is being used to address fundamental ecological and evolutionary questions, such as how species in plant communities are assembled. The process of DNA barcoding entails two basic steps: (1) building the DNA barcode library of known species and (2) matching the barcode sequence of the unknown sample against the barcode library for identification. Although DNA barcoding as a methodology has been in use for less than a decade, it has grown exponentially in terms of the number of sequences generated as barcodes as well as its applications. This volume provides the latest information on generating, applying, and analyzing DNA barcodes across the Tree of Life from animals and fungi to protists, algae, and plants.
推荐方法
- Detection of Transcription Factor Partners with a Yeast One Hybrid Screen
- Sequential Chromatin Immunoprecipitation Protocol: ChIPreChIP
- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis
- Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity
- RNAi in the Malaria Vector, Anopheles gambiae
- Growing Arabidopsis In Vitro: Cell Suspensions, In Vitro Culture, and Regeneration
- Duplicate Ditag Analysis in LongSAGE
- Assembling Large DNA Segments in Yeast
- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos
- Detection and Quantification of Alternative Splicing Variants Using RNA-seq