实验方法>
遗传学实验技术>
基因表达差异显示实验>Cell-Free Protein Synthesis as a Promising Expression System for Recombinant Proteins
Cell-Free Protein Synthesis as a Promising Expression System for Recombinant Proteins
关键词: cell-free protein synthesis来源: 互联网
Cell-free protein synthesis (CFPS) has major advantages over traditional cell-based methods in the capability of high-throughput protein synthesis and special protein production. During recent decades, CFPS has become an alternative protein production platform for both fundamental and applied purposes. Using Renilla luciferase as model protein, we describe a typical process of CFPS in wheat germ extract system, including wheat germ extract preparation, expression vector construction, in vitro protein synthesis (transcription/translation), and target protein assay.
推荐方法
- Family-Based Association Studies
- Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR
- Saturation Mutagenesis
- Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays
- Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview
- Detection and Isolation of Selected Genes of Interest from Metagenomic Libraries by a DNA Microarray Approach
- Probing RNA Folding by Hydroxyl Radical Footprinting
- Optical Mapping of ProteinDNA Complexes on Chromatin Fibers
- Methods for the Study of Ancient DNA
- Isolation of Total RNA and Detection Procedures for miRNA Present in Bovine-Cultured Adipocytes and Adipose Tissues