实验方法>
遗传学实验技术>
基因表达差异显示实验>PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example
PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example
关键词: pcr amplification sequence analysis gc-rich gc rich sequences aristaless-related aristaless related homeobox example来源: 互联网
PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.
推荐方法
- Microsphere-Based Single Nucleotide Polymorphism Genotyping
- Down-Regulation of Gene Expression by RNA-Induced Gene Silencing
- SNP Discovery in Non-model Organisms Using 454 Next Generation Sequencing
- Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) NativeNative
- SIRPH: An HPLC-Based SNuPE for Quantitative Methylation Measurement at Specific CpG Sites
- Using Pyrosequencing to Measure Allele-Specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences
- Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
- Candidate Gene Association Studies
- Tangled Trees: The Challenge of Inferring Species Trees from Coalescent and Noncoalescent Genes
- Mycosis Fungoides and Szary Syndrome