实验方法>
遗传学实验技术>
基因表达差异显示实验>Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
关键词: construction plasmids来源: 互联网
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.
推荐方法
- Plasmid DNA Supercoiling by DNA Gyrase
- Peptide Mass Fingerprinting: Protein Identification Using MALDI-TOF Mass Spectrometry
- Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries
- Transgenic Animals and the Study of Cancer
- Delivery of DNA to Skin by Particle Bombardment
- Establishing Substantial Equivalence: Transcriptomics
- Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield
- Capillary Electrophoresis of mtDNA Cytochrome b Gene Sequences for Animal Species Identification
- Detection and Quantification of Alternative Splicing Variants Using RNA-seq
- Refinement, Reduction, and Replacement