Protein Binding Microarrays (PBMs) for Rapid, High-Throughput Characterization of the Sequence Specificities of DNA Binding Prot
DNA binding proteins play a number of key roles in cells,in processes including transcriptional regulation,recombination,genome rearrangements,and DNA replication, repair,and modification.Of particular interest are the interactions between transcription factors and their DNA binding sites,as they are an integral part of the transcriptional regulatory networks that control gene expression.Despite their importance,the DNA binding specificities of most DNA binding proteins remain unknown,as earlier technologies aimed at characterizing DNA-protein interactions have been time consuming and not highly scalable.We have developed a new DNA microarray-based technology,termed protein binding microarrays (PBMs),that allows rapid,high-throughput characterization of the in vitro DNA binding site sequence specificities of transcription factors in a single day. The resulting DNA binding site data can be used in a number of ways,including for the prediction of the genes regulated by a given transcription factor,annotation of transcription factor function,and functional annotation of the predicted target genes.
- 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities
- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in C
- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection
- Sequencing DNA Amplified Directly from a Bacterial Colony
- Application of Intronic MicroRNA Agents in Cosmetics
- Assaying DNA Topoisomerase I Relaxation Activity
- Bioinformatics Tools for Modeling Transcription Factor Target Genes and Epigenetic Changes
- Gene Expression Profiles and Prognostic Markers for Primary Breast Cancer
- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins
- Production of Chimeras Derived from Murine Embryonic Stem Cells