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基因表达差异显示实验>Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines
Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines
关键词: construction chromosome来源: 互联网
The Yeast Artificial Chromosome (YAC) (1 ) technology allows the cloning of large fragments of DNA (several hundreds of kilobase pairs, kbp) into the budding yeast Saccharomyces cerevisiae , and therefore bridges a gap between conventional cloning in bacteria (λ and cosmid cloning) and somatic cell hybridization techniques. This powerful cloning system has been used successfully for the construction of representative human (2 –5 ), mouse (5 –5 ), Drosophila (8 ), and Caenorrhabditis elegans (9 ) genomic libraries.
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