Large-Scale Production of Membrane Proteins in Pichia pastoris: The Production of G Protein-Coupled Receptors as a Case Study
One of the major advantages of using Pichia pastoris is that it is readily adapted to large-scale culture in bioreactors. Bioreactors allow precise regulation of cell growth parameters increasing both yields and reproducibility of the culture. P. pastoris cultures grow to very high cell densities which helps minimise culture volume and facilitates downstream processing of the sample. Here, we provide protocols for the large-scale production of the human adenosine A2A receptor (A2A R) and provide some details of how bioreactor cultures can be used for optimisation of expression of the human dopamine D2 receptor (D2DR).
- GCG: Assembly of Sequences into New Sequence Constructs
- Overexpression and Purification of Bacterial DNA Topoisomerase I
- Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functional Genomic Studies
- Biolistic DNA Vaccination Against Cervical Cancer
- Small Amplified RNA-SAGE
- Hypomorphic Mice
- Design Considerations for Genetic Linkage and Association Studies
- YAC Localization by Fluorescence In Situ Hybridization
- Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration
- Introduction to Genome Biology: Features, Processes, and Structures