实验方法> 遗传学实验技术> 基因表达差异显示实验>Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis

Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis

关键词: novel applications polymerase chain reaction来源: 互联网

DNA fragments from cells that have died throughout the body not only appear in the bloodstream but also cross the kidney barrier into the urine. The relatively low molecular weight (150-200 bp) of this Transrenal DNA should be considered when deciding on methods of isolation and analysis. In particular, if polymerase chain reaction (PCR) is used for amplification and detection of specific sequences, then the reduction of amplicon size will significantly enhance sensitivity. Detection of DNA mutations is also made more difficult by the presence of a large excess of a wild-type allele. Using K-RAS mutations as an example, two ways around this problem—enriched PCR and stencil-aided mutation analysis—are described, based on selective pre-PCR elimination of wild-type sequences.

推荐方法

Copyright ©2007 ANTPedia, All Rights Reserved

京ICP备07018254号 京公网安备1101085018 电信与信息服务业务经营许可证:京ICP证110310号