T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT Synthesis
Mycobacterium tuberculosis (Mtb) remains a major threat to human health worldwide. Although treatment of infection is an important part of tuberculosis control, an improved vaccine is essential for the elimination of this disease. Control of infection with Mtb is dependent on the cellular immune system, which in turn requires an understanding of those antigens that are capable of stimulating CD4+ and CD8+ T-cell responses. Peptide libraries provide a high-throughput system for identifying novel T-cell epitopes. They can also be used to assess the hierarchy of immunodominance of these novel antigens and epitopes that are associated with infection with Mtb. This T-cell-driven means of antigen discovery is well adapted to vaccine development as well as developing the tools necessary to understand the natural history of this important human pathogen.
- Enzyme-Prodrug Systems: Thymidine Phosphorylase/5-Deoxy-5-Fluorouridine
- SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing
- Preparation of HeLa Nuclear Extracts
- Methods to Analyze microRNA Expression and Function During Xenopus Development
- Suppression Subtractive Hybridization
- HLA Typing by Direct DNA Sequencing
- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides
- A Primer on the Visualization of Microarray Data
- In Vivo Methods to Assess Polyadenylation Efficiency
- Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Line