Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments
关键词: discontinuous polyacrylamide gel electrophoresis来源: 互联网
At present, the most polymorphic genetic markers are DNA regions composed of a variable number of tandem repeats (VNTRs) (1 ,2 ). The detection of VNTRs is made possible by restriction fragment-length polymorphism (RFLP) analysis via Southern blotting (3 ). The length of each DNA fragment is a function of the number of tandem repeats contained within it. The RFLP technique requires at least 10–50 ng of essentially undegraded DNA (10,000–20,000 bp) and isotopic detection to obtain a result; the time required for analysis may be in excess of one week (4 ).
推荐方法
- Single Primer-Mediated Polymerase Chain Reaction
- In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells
- Directional Restriction Site-Free Insertion of PCR Products into Vectors
- Insertional Mutagenesis of the Mouse Germline With Sleeping Beauty Transposition
- An Introduction to the Lagan Alignment Toolkit
- Chromatin Immunoprecipitation Analysis of Xenopus Embryos
- Adjuvants for Plasmid DNA Vaccines
- Comparison of Requirements and Capabilities of Major Multipurpose Software Packages
- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes
- In Vivo Analysis of RNA Editing in Plastids