实验方法> 遗传学实验技术> 基因表达差异显示实验>A Bacterial/Yeast Merged Two-Hybrid System: Protocol for Yeast Screening With Single or Parallel Baits

A Bacterial/Yeast Merged Two-Hybrid System: Protocol for Yeast Screening With Single or Parallel Baits

关键词: bacterial yeast merged来源: 互联网

The yeast two-hybrid system is a useful tool for identifying new protein-protein interactions, and for the dissection of previously identified interactions. An important issue in protein-interaction studies is frequently that of determining whether a protein associates specifically with one protein or domain of interest, or has a more promiscuous interaction profile. To help address this issue, the authors have created a new two-hybrid system, which can be used either in bacteria or in yeast to counterscreen against “decoy” baits in parallel with a primary screen, hence improving the power and specificity of the method. Protocols of this system for use in yeast are provided; a companion article, Serebriiski et al., describes alternative use of this system in bacteria.

推荐方法

Copyright ©2007 ANTPedia, All Rights Reserved

京ICP备07018254号 京公网安备1101085018 电信与信息服务业务经营许可证:京ICP证110310号