Genetic Adjuvants
In 1992, the era of DNA vaccines began with the report of antibody production upon intradermal injection of mice with a plasmid vector expressing a foreign antigen (1 ). A rapid succession of subsequent manuscripts showed stimulation of immune responses, including cytolytic T cells, upon inoculation of expression-vectors specific for antigens derived from viruses, bacteria, protozoa and tumor-associated antigens (2 –7 ). Plasmid DNA can be applied through various routes of injection including: intradermal, intramuscular, subcutaneous, intravenous, or directly on mucosal membranes (1 ,2 ,8 ,9 ). The most commonly used methods of inoculation involve the use of DNA-coated gold beads propelled into the skin by a gene gun or intramuscular inoculation of the vector in saline solution.
- Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies
- siRNA Delivery In Vivo
- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Lig
- Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes
- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12
- Animal Models for Prenatal Gene Therapy: The Nonhuman Primate Model
- Fluorescent Sequencing Protocols for the ALF
- Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
- Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection
- Biolistic Transformation of Brugia Malayi