Microsatellite Analysis of Malaria Parasites
关键词: microsatellite analysis malaria来源: 互联网
Microsatellites have been increasingly used to investigate the population structure of malaria parasites, to map genetic loci contributing to phenotypes such as drug resistance and virulence in laboratory crosses and genome-wide association studies and to distinguish between treatment failures and new infections in clinical trials. Here, we provide optimized protocols for genotyping highly polymorphic microsatellites sampled from across the genomes of the human malaria parasites Plasmodium falciparum and P. vivax that have been extensively used in research laboratories worldwide.
推荐方法
- Quinolone Interactions with DNA and DNA Gyrase
- Sequencing Double-Stranded Linear DNA with Sequenase and [α-35S] d.ATP
- Extraction and Enzymatic Amplification of DNA from Paraffin-Embedded Specimens
- Simple Sequence Repeats Amplification
- Melting Curve Assays for DNA Methylation Analysis
- A Single-Tube Nested RT-PCR for the Detection of Ross River Virus
- PCR Approaches to DNA Mutagenesis and Recombination: An Overview
- Lymphocytes
- Uranyl Photofootprinting
- The Use of Lentiviral Vectors to Obtain Transgenic Rats