Master Regulators of Posttranscriptional Gene Expression Are Subject to Regulation
MicroRNAs (miRNAs) are small noncoding RNAs of 17–25 nt in length that control gene expression posttranscriptionally. As master regulators of posttranscriptional gene expression, miRNAs themselves are subject to tight regulation at multiple steps. The most common mechanisms include miRNA transcription, processing, and localization. Additionally, intricate feedback loops between miRNAs and transcription factors result in unidirectional, reciprocal, or self-directed elegant control mechanisms. In this chapter, we focus on the posttranscriptional regulatory mechanisms that generate miRNAs whose sequence might be slightly different from the miRNA-coding sequences. Hopefully, this information will be helpful in the discovery of novel miRNAs as well as in the analysis of deep-sequencing data and ab initio prediction of miRNAs.
- Global DNA Methylation Analysis Using the Luminometric Methylation Assay
- Footprinting DNAProtein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-PhenanthrolineCopper
- Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV
- Use of Manipulated Stem Cells for Prenatal Therapy
- Isolation of CpG Islands Using a Methyl-CpG Binding Column
- Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse
- The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini
- Preparation of Long Templates for RNA In Vitro Transcription by Recursive PCR
- Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage
- Introduction to Genome Biology: Features, Processes, and Structures