Anesthetizing Mice
关键词: anesthetizing mice来源: 互联网
Operations, such as vasectomy (see Chapter 15 ) and oviduct transfer (see Chapter 20 ), are performed on anesthetized animals. It is essential that the experimenter is familiar with this procedure and that the anesthetic has been tested: A lethal dose of anesthetic administered prior to an oviduct transfer is an unfortunate end to a day spent collecting and microinjecting eggs. The anesthetic described here is Avertin (administered ip); other anesthetics, perhaps routinely used in the experimenter's animal house, may be used.
推荐方法
- GCG: Preparing Sequence Data for Publication
- Medium-Throughput Production of Recombinant Human Proteins: Ligation-Independent Cloning
- Chromosome Banding: Stain Combinations for Specific Regions
- Bacteriophage -Based Expression Vectors
- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
- Microparticle Delivery of Plasmid DNA to Mammalian Cells
- Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
- Qualitative and Quantitative Genotyping Using Single Base Primer Extension Coupled with Matrix-Assisted Laser Desorption/Ionizat
- Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis
- Reducing Sample Complexity in Proteomics<br>by Chromatofocusing with Simple Buffer Mixtures