Strategies to Adapt Adenoviral Vectors for Targeted Delivery
One of the challenges of current gene therapy vector development, concerns targeting a therapeutic gene to diseased cells with the aim of achieving sufficient gene expression in the affected tissue, while minimizing toxicity and expression in other tissues. The use of recombinant adenoviruses as vectors for gene therapy is restricted by the widespread distribution of the coxsackie and adenovirus receptor (CAR) (1 ,2 ), which allows infection of a range of tissues and precludes specific in vivo targeting. It is now well accepted that there is a dose-dependent toxicity associated with systemic delivery of adenoviral (Ad) vectors, in particular the risk of hepatotoxicity is a major concern (3 –6 ). Thus, development of Ad vectors that can target specific tissues following systemic or minimally invasive administration would enhance their therapeutic potential and expand their application. Targeting can be achieved at the level of capsid binding or at later transduction events by the use of tissue-specific promoters (7 –10 ). Targeting at the level of binding is preferred because even the interaction of cells with empty capsids leads to toxic effects (11 ), however a combination of both strategies has its obvious advantages.
- Site-Directed Cleavage of DNA by ProteinFe(II) EDTA Conjugates Within Model Chromatin Complexes
- Analysis of DNA Restriction Enzyme Digests by Two-Dimensional Electrophoresis in Agarose Gels
- A Microtiter-Plate-Based High Throughput PCR Product Purification Method
- Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets
- Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems
- The Use of Flow Cytometry to Detect Transfected Gene Products
- Sequence Databases and the Internet
- Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors
- M13 Cloning Vehicles: Their Contribution to DNA Sequencing
- Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics