Transgenic Studies in the Mouse: Improving the Technology Towards a Conditional Temporal and Spatial Approach
The biological consequences of altered gene expression as a result of gain of function mutations or gene dosage remains an important question in molecular biology and molecular medicine. This is of particular interest in medical research due to the many clinically relevant diseases that involve altered gene expression. The biology of cancer is one such example whereby a spectrum of genes may be inappropriately activated or inactivated contributing to disease progression. These genes provide ideal and direct targets for the development of cancer therapeutics and emphasize the requirement for transgenic mice as in vivo disease models. Furthermore, the inappropriate expression of transcription factor encoding genes may lead to dysregulation of gene expression and significant biological consequences. These questions can only be addressed by using technical strategies that allow the introduction or activation of a test protein in a given spatial and temporal manner. New technologies are continually being sought in order to generate sophisticated animal models that appropriately reflect the genetic basis of the disease of interest. These animals have the added benefit of being invaluable in drug discovery (1 ).
- Subtractive cDNA Cloning Using Magnetic Beads and PCR
- Array-Based Approaches in Prenatal Diagnosis
- High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae
- Targeting Integration of the Saccharomyces Ty5 Retrotransposon
- Native/Denaturing Two-Dimensional DNA Electrophoresis and Its Application to the Analysis of Recombination Intermediates
- Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning
- Nearest-Neighbor Analysis
- The Importance of Mouse ES Cell Line Selection
- Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System
- Engineering Cys2His2 Zinc Finger Domains Using a Bacterial Cell-Based Two-Hybrid Selection System