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基因表达差异显示实验>A Strategy for Identifying Noncoding RNAs Using Whole-Genome Tiling Arrays
A Strategy for Identifying Noncoding RNAs Using Whole-Genome Tiling Arrays
关键词: strategy identifying来源: 互联网
Whole-genome tiling arrays are powerful tools for detecting and characterizing novel RNA transcripts. Here, we describe a complete method combining elements of molecular and computational biology to identify small noncoding RNA (sRNA) transcripts. We focus on the key features of this approach, which include size-fractionation of input RNA, direct detection of array hybridization with antibodies that recognize RNA:DNA hybrids, and correlation-based computational methods for automated sRNA identification and boundary determination.
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