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Isolation of cDNAs Using the YAC Hybridization Screen Method
关键词： isolation cdnas来源： 互联网
There are currently a number of techniques available for the isolation of novel coding sequences from cloned genomic fragments. The most recent methodologies include exon trapping (1 ), direct selection (2 ,3 ), and screening of cDNA libraries with whole radiolabeled yeast artificial chromosomes (YACs) (YAC hybridization) (4 ).
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