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基因表达差异显示实验>Application of In-Fusion Cloning for the Parallel Construction of E. coli Expression Vectors
Application of In-Fusion Cloning for the Parallel Construction of E. coli Expression Vectors
关键词： application in-fusion cloning来源： 互联网
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.
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