Viral Small RNA Cloning and Sequencing
At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host–pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).
- Staden: Comparing Sequences
- MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs
- The Therapeutic Potential of LNA-modified siRNAs: Reduction of Off-target Effects by Chemical Modification of the siRNA Sequence
- Global Strain Engineering by Mutant Transcription Factors
- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants: An Overview
- Complementary Proteomic Analysis of Protein Complexes
- Construction of Improved Yeast Two-Hybrid Libraries
- Applications of Array-CGH for Lung Cancer
- Genome Engineering Using Targeted Oligonucleotide Libraries and Functional Selection
- Development of DNA Vaccines for Salmonid Fish