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基因表达差异显示实验>Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
关键词: simple efficient identification来源: 互联网
Affinity purification and mass spectrometry analysis have been used to identify and characterize protein complexes. Wdr82-associated chromatin modifying complexes were purified by single-step FLAG affinity purification from human cells induced to express FLAG-tagged Wdr82. Purified proteins were analyzed by SDS-PAGE and specific protein bands were identified by mass spectrometry. Subsequently, purified proteins were fractionated on sucrose gradient equilibrium centrifugation to determine overall composition of each identified complex. We describe here simple and efficient approaches for the identification of chromatin modifying complexes and subsequent characterization of complex composition.
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