Analysis of the Impact of Genetic Variation on Human Gene Expression
Interindividual variation in gene expression has been convincingly shown to be controlled, in part, by genetic differences. Determining the architecture of genetic variation, the underlying gene expression may allow deeper insight into complex phenotypes, such as differences in disease susceptibility. Mapping genetic variants accounting for expression phenotypes in human cell and tissue panels has rapidly progressed from proof-of-principle experiments to general tools in biomedical discovery. We discuss the general approach and critical considerations for carrying out expression quantitative trait mapping in human tissues.
- Nucleic Acid Transfer Using Cationic Lipids
- High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli
- Analysis of Allele-Specific Gene Expression Using a Target-Oriented Tiling Microarray Assay
- Positive Selection Scanning of Parasite DNA Sequences
- Assays for Structure-Selective DNA Endonucleases
- Clone Excision Methods for the Lambda ZAP-Based Vectors
- Computational and Bioinformatics Methods for MicroRNA Gene Prediction
- Analysis of Fusion Using a Virus-Free Cell Fusion Assay
- Ethical Ramifications of Genetic Analysis Using DNA Arrays
- Fabrication of cDNA Microarrays