The ForeSee (4C) Approach for Integrative Analysis in Gene Discovery
The development of high-throughput experimental techniques has made measurements for virtually all kinds of cellular components possible. Effective integration and analysis of this diverged information to produce insightful knowledge is central to biological study today. In this chapter, we present a methodology for building integrative analytical workbenches using the workflow technology. We focus on the field of gene discovery through the combined study of transcriptomics, genomics and epigenomics, although the methodology is generally applicable to any omics- data analysis for biomarker discovery. We illustrate the application of the methodology by presenting our study on the identification of aberrant genomic regions, genes and/or their regulatory elements with their implications for breast cancer research. We also discuss the challenges and opportunities brought by the latest development of the next generation sequencing technology.
- 外源基因在原核和真核细胞的表达(一)
- Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases
- Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)
- Microchip-Based Enumeration of Human White Blood Cells
- Biochemical Analysis of Long Terminal Repeat Retrotransposons
- Predicting the Functional Consequences of Somatic Missense Mutations Found in Tumors
- Manual Solid Phase Synthesis of Glutathione Analogs: A Laboratory-Based Short Course
- Virus-Aided Gene Expression and Silencing Using TRV for Functional Analysis of Floral Scent-Related Genes
- Naked DNA Gene Transfer in Mammalian Cells
- Visualization of DNA Replication Sites in Mammalian Nuclei