The genetic dissection of complex disorders via genetic marker data has gained popularity in the postgenome era. Methods for typing genetic markers on human chromosomes continue to improve. Compared with the popular individual genotyping experiment, a pooled-DNA experiment (alleotyping experiment) is more cost effective when carrying out genetic typing. This chapter provides an overview of association mapping using pooled DNA and describes a five-stage study design including the preliminary calibration of peak intensities, estimation of allele frequency, single-locus association mapping, multilocus association mapping, and a confirmation study. Software and an analysis of authentic data are presented. The strengths and weaknesses of pooled-DNA analyses, as well as possible future applications for this method, are discussed.