Label-Free Detection with the Resonant Mirror Biosensor
关键词: resonant mirror biosensor来源: 互联网
The resonant mirror (RM) biosensor is a leaky waveguide-based instrument that uses the evanescent field to probe changes in the refractive index at the sensing surface.The RM can therefore be used to monitor in real-time and label-free the interaction between an analyte in solution and its biospecific partner immobilized on the waveguide surface.The RM has been used in studying the interaction of a variety of moieties including proteins, carbohydrates, cells, nucleic acids and receptors, leading to applications in areas such as clinical diagnostics, homeland security, and pharmaceutical and biomolecular interactions. This chapter will review the principle of this biosensor, and the recent advances in instrumentation, different immobilization chemistries, and kinetic studies, as well as some applications.
推荐方法
- Fluorescence Lifetime-Based Optical Molecular Imaging
- Electron Microscopic Enzyme Cytochemistry
- Microscopy with Oil Immersion
- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
- Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology
- 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)
- Determination of Absolute Molecular Weights Using Sedimentation Equilibrium Analytical Ultracentrifugation
- Single mRNP Tracking in Living Mammalian Cells
- 荧光寿命( FLT)检测
- 荧光显微镜原理