BIELSCHOWSKY METHOD
关键词: bielschowsky method来源: 互联网
SECTIONS 8micron paraffin wax sections.
SOLUTIONS
1. Silver A - 20% silver nitrate
2. Reducer (Make fresh)
- Formaldehyde 50ml
- Absolute Alcohol 200ml
- Distilled water 750ml
3. Silver B
- 20% Silver nitrate 20ml
- 95% Alcohol 20ml
- Add ammonia until precipitate formed is redissolved
- Add 10 more drops of ammonia
4. 5% Sodium thiosulphate5. 0.1% Gold chloride
METHOD
Take sections to distilled water Place in solution A for 2-3 hours Wash in reducer for 2-5 minutes Wash in 10% alcohol for 2 minutes Place in solution B for 1-2 minutes Wash in a constant flow of reducer and leave for 3-5 minutes Wash in distilled water Tone 0.1% gold chloride for 2 minutes Wash in distilled water Fix in 5% sodium thiosulphate for 2 minutes Wash in tap water Dehydrate, clear . Mount sections in DPX
RESULTS Neurites around senile plaques, neurofibrillary tangles - Black.
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