Detection of Cell Death in Neural Tissue and Cell Culture
In recent years, we have studied cell death in the developing nervous system (Owens et al., 1995), in fetal tissue grafts (Mahalik et al., 1995), and in the rodent olfactory epithelium (Mahalik, 1995). In addition, we are interested in correlating cell death in the rodent CNS with the expression of a putative death-related mRNA called RPS (Owens et al., 1991; Owens et al., 1995; Mahalik et al., 1995). In these studies we have used standard Nissl stains; fluorescent dyes, such as Hoechst 33258 which stain DNA; and the TUNEL method (Gavrieli et al., 19921, in which biotinylated deoxyUTP is incorporated into -hydroxyl groups in nicked or fragmented DNA to identify apoptotic cells in tissue sections. In more recent work, we have combined the TUNEL procedure with immunocytochemistry to identify dying cells in the rodent olfactory epithelium (Mahalik, 1995). Finally, we have used modifications of the TUNEL procedure and other methods to study the aspects of cell death and apoptosis in cultures of PC12 cells and immature rodent thymocytes.
- General Introduction to In Situ Hybridization Protocol Using Nonradioactively Labeled Probes to Detect mRNAs on Tissue Sections
- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
- Animal Models of Diabetic Neuropathic Pain
- Production of Genetically Engineered Cells Releasing Neurotrophic Factors
- Determination of Histamine in Microdialysis Samples from the Rodent Brain by Column Liquid Chromatography
- G Proteins and Animal Models of Parkinsons Disease
- Primary Cultures of Astrocytes from Fetal Bovine Brain
- Recording Currents from Channels and Transporters in Macropatches
- Habit Formation and Compulsion
- Axonal Transport Methods and Applications