In ovo electroporation is a popular technique to study gene function during development. This technique enables precise temporal and spatial genetic manipulation with the added advantages of being quick and inexpensive. In this chapter the transient transfection of a construct into the neural tube of a chicken embryo via in ovo electroporation is described. Modifications of this basic technique and methods to �analyze the resulting electroporated embryos such as qPCR and microarray are also discussed.