In retinal diseases characterized by photoreceptor degeneration, the main cause of clinically significant vision loss is cone, rather than rod, loss. Photoreceptor apoptosis can be affected by the availability and/or delivery of neurotrophic proteins, and cultures of photoreceptors are valuable for studying these processes. In the present study, a technique was designed to purify cones to make it possible to screen for neuroprotective molecules. The present chapter describes a technique for preparing cultures of purified rat retina cone photoreceptors based upon panning with peanut agglutinin lectin, which selectively binds to cones. In addition, we describe a protocol for the purification and culture of retinal pigmented epithelial cells from postnatal rat.