Studying M1 and M2 States in Adult Microglia
Microglial cell function receives increasing interest. To date, the majority of experiments are performed by using immortalized microglia-like cells or primary microglia prepared from pre- or postnatal rodent brain. As those may not adequately reflect the microglial biology in the adult brain, this protocol advocates a procedure which allows for the isolation, purification, and subsequent analysis of microglial cells. Once isolated, the principal state of activation, M1 or M2, can be determined in adult microglia using fluorescence-activated cell sorting, quantitative PCR, and/or Western blotting. Likewise, adult microglia generated by this protocol can be used for functional analysis through cell cultivation for a limited time.
- The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences
- Brain Mapping with High-Resolution fMRI Technology
- Patch-Clamp and Single-Cell Reverse TranscriptionPolymerase Chain Reaction/Microarray Analysis
- Radioligand Binding Studies: Pharmacological Profiles of Cloned Y-Receptor Subtypes
- Animal Models of Diabetic Neuropathic Pain
- Protein ADP-Ribosylation
- In Vivo Optical Recording of Brain Interneuron Activities from a Drosophila Male on a Treadmill
- Principles of Single-Channel Kinetic Analysis
- In Vivo Animal Models of Teratogenicity
- A Brief Overview of Multitalented Microglia