Quantitative In Vivo Microdialysis in Pharmacokinetic Studies
Recent theoretical studies have yielded a more profound knowledge of the properties of recovery (the key parameter in quantitative microdialysis) and have put in evidence important limitations of the usual in vivo calibration methods used in quantitative microdialysis for pharmacokinetic studies. Recovery values obtained by using the more classical methods of calibration (the variation of flow rate perfusion method, the delivery and retrodialysis methods, and the no net flux method) can only be used to accurately convert dialysate drug concentrations into extracellular concentrations, when the drug of interest is in the body under steady-state conditions. Therefore, these in vivo calibration procedures must not be used when the drug studied has to be administered using modalities of administration which do not provide steady-state concentrations (for example, intragastric, subcutaneous, intraperitoneal, or intravenous bolus injections). The dynamic no net flux (DNNF) method, however, can be considered the only in vivo calibration method useful in PK experiments developed under transient conditions, although this calibration procedure has several serious disadvantages. The new modified version of the ultraslow microdialysis (the MetaQuant technique) overcomes many of the limitations of both the classical calibration and the DNNF methods and, therefore, it could be considered a promising tool in pharmacokinetics.
- Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
- Introduction to Part I: Caged Neurotransmitters
- High-Throughput Functional Detection of NMDA Receptor Activity
- Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood-Brain Barrier Permeability to Contrast Agents
- Patch-Cram Detection of Cyclic GMP in Intact Cells
- Autonomic Nervous System In Vitro: Studying Tonically Active Neurons Controlling Vagal Outflow in Rodent Brainstem Slices
- Live Imaging of Neural Cell Functions
- Immunoblot Detection of Brain Vascular Proteins
- Cells from Testis for Transplantation in the CNS
- Neurological Evaluation of Movement Disorders in Mice