Convection-enhanced drug delivery is a novel technology used to deliver drugs into brain tissue and which is currently evaluated in clinical trials. Drugs are delivered continuously via intracranial catheters and enable to achieve large volume of distributions at high drug concentrations with minimum systemic toxicity. Accumulated clinical experience has shown that convection does not always form and the efficiency of distribution varies among patients and different drugs. The efficiency of drug distribution depends on multiple treatment and physiological parameters, such as infusate characteristics, catheter type, flow rate, volume of distribution, and tissue characterization along the catheter path and at the catheter tip. Therefore, extensive research is ongoing focusing on optimizing convection to enhance the therapeutic effect of drugs in various brain disorders. This chapter describes various aspects of performing convection-enhanced drug delivery experiments in a rat brain model.