An Overview of In Vitro Techniques for Blood-Brain Barrier Studies
The first successful isolation of cerebral microvessels from rat brain some 30 years ago opened the way to the development of in vitro models of the blood-brain barrier (BBB), which have since contributed considerably to the understanding of the physiology, pharmacology, and pathophysiology of the BBB (1 ). However, the ever increasing number of in vitro models and techniques also creates problems that make transferability of results obtained with different models difficult even for experts, while scientists new to the field often find it hard to choose an optimal model for their particular interests. This chapter gives a brief overview of the current status of in vitro BBB models currently used and provides some guidance through the host of models with regard to their suitability for specific applications.
- Analysis of Mitochondrial DNA Mutations
- High-Throughput Functional Detection of NMDA Receptor Activity
- Microdialysis and Advances for Sampling Synaptic and Extrasynaptic Pools
- Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish
- Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents
- Synthesis of Conformationally Restricted Peptides
- In Vivo Optical Recording of Brain Interneuron Activities from a Drosophila Male on a Treadmill
- Stable Isotope Labeling with Amino Acids in Cell Culture-Based Proteomic Analysis of Ethanol-Induced Protein Expression Profiles
- Histological Assessment of Angiogenesis in the Hypoxic Central Nervous System
- 日研究蓝光可促进人造血管增厚