Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man
This chapter presents fast and easy protocols to obtain highly purified cultures of proliferating adult rat, canine, and human Schwann cells. Cell preparation from predegenerated adult sciatic nerves combined with the use of melanocyte growth medium supplemented with forskolin, fibroblast growth factor-2, pituitary extract, and heregulin as selective, serum-free culture medium and two methods for a consecutive cell-enrichment step are described. Our protocols result in approximately 90% pure Schwann cell cultures (or higher). The average time to obtain highly purified in vitro cultures of adult Schwann cells is 21 days.
- Retrograde Axonal Tract Tracing
- The Design and Use of Oligonucleotides
- Purification of Endothelial Cells from Rat Brain
- Forelimb Functional Assessments of Rats and Mice After Cervical Spinal Cord Injury
- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination
- Behavioral Phenotyping of Mouse Models of Neurodegeneration
- Neurotransmitter Transporters and Anticonvulsant Drug Development
- Quantification of mRNA Levels Using Northern Blotting
- Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry
- Introduction: Anorexia and Undereating