实验方法>
实验仪器使用技术>
神经生物学>Sindbis Virus-Mediated In Vivo Expression of Recombinant CREB to Study Gene Function in Neuronal Plasticity and Behavior
Sindbis Virus-Mediated In Vivo Expression of Recombinant CREB to Study Gene Function in Neuronal Plasticity and Behavior
关键词: sindbis virus-mediated expression来源: 互联网
Understanding the role of specific genes in diverse brain functions is a major challenge in neuroscience. To address this issue, we have optimized an in vivo transgene expression system based on the sindbis virus. In this chapter, we provide a detailed description of the cloning, production, and in vivo stereotaxic injection of sindbis viral particles expressing proteins of interest. As an example, we describe how this technique can be used to identify the roles of the transcription factor, cAMP-responsive-element binding protein, in the regulation of neuronal plasticity and memory formation.
推荐方法
- Production of Antisera Using Peptide Conjugates
- Retinal Inflammation: Uveitis/Uveoretinitis
- G Proteins in the Medial Temporal Lobe in Schizophrenia
- The Behavioral Repertoire of Larval Zebrafish
- Combining Patch-Clamp Techniques with RT-PCR
- Experimental Systems to Study Neurotrophic Factor Effects on Rat Brain Cells
- Studying Schizophrenia in a Dish: Use of Primary Neuronal Cultures to Study the Long-Term Effects of NMDA Receptor Antagonists o
- Investigation of Astrocyte Oligodendrocyte Interactions in Human Cultures
- Validation of Dementia Models Employing Neuroimaging Techniques
- Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy of Cell-Penetrating Neuropeptide