Light Sheet Microscopy in Cell Biology
Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications. Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection.
- Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy
- MRI of CEST-Based Reporter Gene
- In Vitro Selection of Protein-Binding DNA Aptamers as Ligands for Biosensing Applications
- Magnetic Resonance Imaging of Tumor Physiology
- FDG PET Imaging of Head and Neck Cancers
- Correlative Microscopy of Individual Cells: Sequential Application of Microscopic Systems with Increasing Resolution to Study th
- Magnetic Resonance Safety
- Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors
- AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation
- Fluoroimmunoassays Using the NRL Array Biosensor