Genomic DNA Extraction for Mapping实验方法详情页

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Genomic DNA Extraction for Mapping

关键词： DNA Extraction Plants DNA提取植物来源：互联网

Solutions:

Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS

Extraction Buffer

1 M Tris-HCl pH 7.5	10.00 ml
5 M NaCl	2.50 ml
0.5 M EDTA	2.50 ml
20% SDS	1.25 ml
H 2 O	33.75 ml
Total	50.00 ml

Remarks:

This protocol is quick, reliable and the DNA obtained can be used for PCR-based mapping using SSLP and CAPS markers. It is based on the protocol by Edwards et.al., 1991, NAR 19: 1349. It was adapted for large-scale applications by the Somerville lab.

Materials:

Reagent/Tool	Supplier	Cat.-#

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