Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains
Single-strand conformation polymorphism (SSCP) analysis is a sensitive and rapid technique for detecting DNA polymorphisms and mutations in PCR-amplified fragments. Due to its technical simplicity, it is widely used as a screening tool in various investigations, ranging from clinical diagnosis of human hereditary diseases to the characterization of microbial communities. This method can also be used successfully on phytoplasmas as a tool for the detection of molecular variability in conserved housekeeping genes such as 16S rRNA and tuf , as well as in more variable genes, revealing the presence of polymorphisms undetected by routine RFLP analyses. The reliability of SSCP has been confirmed by multiple alignments and phylogenetic analyses of representative sequences showing different SSCP profiles. However, it is not broadly applied in phytoplasma research yet. The technique provides an inexpensive, convenient, and sensitive method for determining sequence variation and to differentiate phytoplasma strains, and is particularly suitable for epidemiological studies or as a fast screening, typing tool when dealing with a large number of field samples.
- 麒麟尾
- 维管束(vascular bundle)
- 盐碱地
- Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls
- Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis
- Immunomodulation of Plant Function by In Vitro Selected Single-Chain Fv Intrabodies
- Comparative Gel-Based Phosphoproteomics in Response to Signaling Molecules
- An Efficient Method for the Micropropagation of Agave Species
- Recombinant Baculoviruses
- Automated Microscopy in Forward Genetic Screening of Arabidopsis