Chromatin Immunoprecipitation to Verify or to Identify In Vivo ProteinDNA Interactions
Chromatin immunoprecipitation (ChIP) is a valuable tool to detect the interaction in vivo between a DNA-associated protein and DNA fragments. Combined with approaches to assess gene expression in response to accumulation of a transcription factor, it is possible to identify direct responsive targets from targets that are indirectly responsive to accumulation of the transcription factor. ChIP may be used to confirm in vivo association of a transcriptional regulator with suspected target DNA fragments. ChIP may also be used to discover new targets, and when combined with high-throughput approaches to identify DNA fragments associated with a transcription factor, it may provide a tool to study the gene regulatory networks active during plant development and/or response to the environment. Furthermore, ChIP is also a powerful means to map epigenetic modifications within a genome.
- 植物可滴定酸的测定
- 质壁分离法测定植物细胞的渗透势
- Lipid-Binding Analysis Using a Fat Blot Assay
- 裸子植物(Gymnospermae)
- 孢子纲(Sporozoa)
- 雌球花
- Functional Genomics and Structural Biology in the Definition of Gene Function
- Mapping Functional Domains of Transcription Factors
- Transcript Profiling and Expression Level Mapping
- Cryopreservation of Plant Cells