Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
关键词: antisense oligodeoxynucleotide-mediated来源: 互联网
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
推荐方法
- 吸胀吸水
- 甲藻门(Pyrrophyta)
- 墨角藻黄素
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