Papaya (Carica papaya L.)
Transgenic papaya plants were initially obtained using particle bombardment, a method having poor efficiency in producing intact, single-copy insertion of transgenes. Single-copy gene insertion was improved using Agrobacterium tumefaciens . With progress being made in genome sequencing and gene discovery, there is a need for more efficient methods of transformation in order to study the function of these genes. We describe a protocol for Agrobacterium -mediated transformation using carborundum-wounded papaya embryogenic calli. This method should lead to high-throughput transformation, which on average produced at least one plant that was positive in polymerase chain reaction (PCR), histochemical staining, or by Southern blot hybridization from 10 to 20% of the callus clusters that had been co-cultivated with Agrobacterium . Plants regenerated from the callus clusters in 9 to13 mo.
- Total Protein Extraction with TCA-Acetone
- Virus-Induced Gene Silencing in Plant Roots
- 旌节花科(Stachyuraceae)
- Chymotrypsin Inhibitor
- Geminivirus: Biolistic Inoculation and Molecular Diagnosis
- Assaying Levels of Virus with Local Lesion Hosts
- Applications of RT-PCR
- Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy
- Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases
- T-DNA Mutagenesis in Arabidopsis