In Silico Methods for Identifying Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Pr
Numerous experimental and in silico approaches have been developed for attempting to identify the �subcellular localisation of proteins. Approximately 2,000–4,000 proteins are thought to be targeted to plastids in plants, but a complete and unambiguous catalogue has yet to be drawn up. This article reviews the various prediction methods that identify plastid targeting sequences, and those that can help estimate location and topology within the plastid or plastid membranes. The most successful approaches are described in detail, with detailed notes to help avoid common pitfalls and advice on interpreting conflicting or ambiguous results. In most cases, it is best to try multiple approaches, and we also cover the powerful new integrated databases that provide a selected blend of experimental data and predictions.
- Liposome-Binding Assays to Assess Specificity and Affinity of PhospholipidProtein Interactions
- 胡萝卜愈伤组织的建立
- 棣棠花
- Batch Suspension Culture
- Mass Spectrometry-Based Imaging of Metabolites and Proteins
- 维管植物(vascular plants)
- Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration
- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants
- 天门冬
- RNA-Seq for Transcriptome Analysis in Non-model Plants