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植物生理>Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons
Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons
关键词: development sequence来源: 互联网
Among the PCR-based markers that are most widely used in molecular genetic studies, SCARs (sequence characterized amplified regions) have the advantage of being less sensitive to the conditions of a standard PCR due to its primer size when compared to RAPD (random amplified polymorphic DNA) and hence are more specific and reproducible. Moreover, SCARs require no radioactive isotopes and detect only a single locus. Here, we describe the development of SCAR from RAPD amplicons.
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