Northern Blotting Techniques for Small RNAs
In eukaryotes, RNA silencing encompasses a range of biochemical processes mediated by ∼20–25 nt small RNAs (smRNAs). This chapter describes northern blot hybridization techniques optimized for detection of such smRNAs, whether extracted from plant or animal tissues. The basic protocol is described, and control blots illustrate the detection specificity and sensitivity of this method using DNA oligonucleotide probes. Known endogenous smRNAs are analyzed in samples prepared from several model plant species, including Arabidopsis thaliana , Nicotiana benthamiana , Oryza sativa , Zea mays , and Physcomitrella patens , as well as the animals Drosophila melanogaster and Mus musculus . Finally, the usefulness of northern blotting in dissecting smRNA biogenesis is shown for the particular case of DNA virus infection.
- 植物体内游离氨基酸总量的测定
- 胚芽鞘
- 子囊茵亚门(Ascomycotina)
- 烟草(Nicotiana tabacum)
- 筛孔
- 青贮饲料
- Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela odorata)
- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy
- 谷子
- EMS Mutagenesis of Arabidopsis